Muscle fiber typing is conventionally performed using mATPase enzyme histochemistry on cryostat sections.
Muscle fiber profile (SO = slow oxidative fiber; red fiber; type I, FOG = fast oxido-glycolytic fiber; intermediate; type IIA and FG = fast glycolytic fiber; white fiber; type IIB)
Both Pectoralis and Bicep Femoris muscles were dissected from each specimen and quickly frozen by whole immersion in isopentane that was cooled in liquid nitrogen. The frozen muscles were wrapped in aluminum foil, placed in sealed plastic bags, and stored at –80°C until they were used for sectioning and staining.
Muscle samples were stored at -80 C, Sectioning muscle samples by cryostat.
Staining slides, mounting slide with coverslip, checking quality before analyzing with the software so called IT3 image analysis.
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